Summary
Overview
Work History
Education
Skills
Languages
Timeline
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Lorena Moran Bombonato

Lorena Moran Bombonato

Maringá, Paraná

Summary

I am a Master's student in Health Sciences at the State University of Maringá (UEM), specializing in clinical microbiology, antimicrobial resistance, and molecular techniques. My research focuses on drug resistance in Mycobacterium tuberculosis, with an emphasis on molecular diagnostics, including DNA extraction and PCR. I have experience in interpreting clinical and laboratory data, as well as writing academic papers. I aim to advance diagnostic methods and contribute to public health, while remaining highly motivated and passionate about learning and applying my knowledge to socially relevant projects.

Overview

3
3
years of professional experience

Work History

Master´s Studant at State University Os Maringá

State University of Maringá
Maringá, Paraná
03.2024 - Current
  • Performing cell culture using J77 macrophage and VERO epithelial cells to support infection, toxicity, and viability assays in antimicrobial studies.
  • Conducting laboratory assays, including the Resazurin Microtiter Assay (REMA) to assess bacterial viability and drug susceptibility.
  • Performing checkerboard assays to evaluate the synergistic effects of drug combinations.
  • Leading efflux pump inhibition assays to investigate resistance mechanisms in Mycobacterium tuberculosis.
  • Extracting DNA from mycobacteria using protocols adapted to sample type and downstream application.
  • Utilizing Genolyse kits (Hain Lifescience), QIAGEN kits, and ethanol precipitation methods for DNA extraction.
  • Performing PCR using Xgen kits for genus- and species-specific identification.
  • Applying MTBDRplus kits (Hain Lifescience) to detect mutations associated with resistance to rifampicin and isoniazid.
  • Conducting DNA hybridization using Line Probe Assay (LPA) for rapid detection of Mycobacterium tuberculosis resistance profiles.

Internship in Clinical Analysis Laboratory

Ingá University (UNINGÁ)
Maringá, Paraná
03.2023 - 12.2023
  • Performed hematological, parasitological, biochemical, and urinalysis tests to support diagnosis of infections, metabolic disorders, and organ dysfunctions.

Blood Collection Internship

Ingá University (UNINGÁ)
Maringá, Paraná
03.2022 - 12.2022
  • Performed blood collection, ensured sample integrity, managed labeling/preparation, and collaborated with healthcare teams to support accurate diagnostics and patient care.

Education

Bachelor of Science - Biomedical Sciences

Ingá University
Maringá, Paraná, Brazil
01-2024

Master of Science - Medical Bacteriology

State University of Maringá
Maringá, Paraná

Skills

  • Cell culture techniques
  • PCR methodologies
  • DNA extraction processes
  • Laboratory assays and testing
  • Drug susceptibility analysis
  • Infection control practices
  • Mycobacterium tuberculosis expertise
  • Teamwork and adaptability
  • Molecular techniques

Languages

Portuguese
First Language
English
Intermediate (B1)
B1
French
Beginner
A1

Timeline

Master´s Studant at State University Os Maringá

State University of Maringá
03.2024 - Current

Internship in Clinical Analysis Laboratory

Ingá University (UNINGÁ)
03.2023 - 12.2023

Blood Collection Internship

Ingá University (UNINGÁ)
03.2022 - 12.2022

Bachelor of Science - Biomedical Sciences

Ingá University

Master of Science - Medical Bacteriology

State University of Maringá

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Lorena Moran Bombonato